All Protocols
These protocols contain everything you need to know to get started with practical Synthetic Biology. You can learn the basic genetic theory that explains why these protocols work by following this link.
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The Graduate’s Guide to Synthetic Biology
Complete List of Protocols:
Water
Tris-Cl Buffer
Agarose Electrophoresis Buffers
TAE Buffer
LAB Buffer
TBE Buffer
Protein Purification Buffers
Protein Wash Buffer
Protein Elution Buffer
Protein Storage Buffer
SDS-PAGE Buffers
SDS-PAGE 4x Loading Buffer
SDS-PAGE 10x Running Buffer
Coomassie Blue Staining Solution
Destaining Solution
Plasmids101 - Guide coming soon!
Preparation of Chemically Competent E.coli cells (Rubidium Chloride) for Heat Shock Transformation
Preparation of Chemically Competent E.coli cells (Calcium Chloride) for Heat Shock Transformation
Preparation of Electrocompetent cells of Pseudomonas or E. coli for Electroporation
Electroporation of Electrocompetent cells of Pseudomonas or E. coli for Electroporation
Restriction Enzyme Digest of Plasmid & Insert DNA, Optional: Dephosphorylation + Purification
Kinase Treatment
Interpreting Sequencing Results
Preparation of Glycerol Stocks
CRISPR/CAS9
Proteins101
Chemical Lysis
Column Chromatography His-Tag Purification
Antibody Production
Aptamer Production
Cardinal Blots
Northern Blot
Eastern Blot
Southern Blot
Western Blot
ELISA
Mass Spec Prep
Cell Assays
RNA101
RNA Purification
RT-PCR
RT-qPCR
In Silica