Northern Blot

 

The Northern blot is an alternative to RT-PCR for the detection of RNA sequences. A denaturing gel is used to separate RNA samples according to size. This gel is transferred to a nylon membrane and labeled with complimentary RNA probes.

Equipment & Consumables:

  • Micropipette and sterile tips

  • 1.5 ml Centrifuge Tubes

  • Tweezers

  • Ice in Ice Bucket

  • Agarose Electrophoresis Gel Tray and Bath

  • DC Power Supply

  • Microwave

  • Centrifuge

  • Thermal Cycler

  • Heat Block

  • Vacuum Gel Transfer System

  • Nylon Membrane

  • UV crosslinker

  • Hybridisation oven

  • Hybridisation bottles

  • Scintillation vial

  • Scintillation counter

  • Phosphor Screen

  • Phosphor Screen scanning equipment

Protocol: