Imaging Enclosure
DIY 3D printed imaging enclosure that I designed in CAD and then 3D printed in PLA plastic. The aluminium foil is almost definitely overkill, but I couldn’t be certain that the plastic was blocking 100% of the UV radiation.
Illuminator is an second-hand Macrovue.
What are they and why do we use them? What types are there?
An imaging enclosure is used to protect you from potentially harmful radiation from your illuminator. It also provides added contrast to images of your gel, allowing you to see the bands far more easily. A good enclosure completely blocks light from entering or leaving the gel imaging stage, with the exception of the camera aperture.
When do you use?
For Gel Illumination and Photography after Agarose Gel Electrophoresis!
How do you use?
Place the gel on the illuminator. Place the enclosure over the top and ensure there are no gaps. Place the camera (smartphone) over the aperture and then turn on the light. Take a few photos, then turn off the light. Check the photos and adjust the gel/zoom/focus as necessary. Once you have a good photo you can throw away the gel and clean all the equipment. Be especially careful to wear gloves if your gels contain Ethidium Bromide.
If you’re using an all-in-one imaging and enclosure system, follow the appropriate manual.